If you try to copy a large file from a network share sometimes Windows Server 2008 or Vista freezes the network connection although you have Service Pack 1 installed. After that you have to reboot in order to get your network connection to work.
You can find some discussions without any solution. I had the same problem and this workaround works for me:

1. Never click at the "More information" button which is calculating the speed. Since I don't look at the information even file copies of 26GB are working - up and down.
2. Copy large files one by one using the crtl+c key combination.
3. Don't drag and drop files or folders.

I hope it works for somebody too.